The eukaryotic cell is compartmentalized by membrane-bounded organelles that carry out linked biochemical reactions that sustain life. We investigate how the activities of the biosynthetic secretory and the endocytic pathways respond to metabolic, physiological, and environmental cues to effect rapid and specific changes in inter-organelle trafficking focusing the on the Golgi apparatus, the plasma membrane, and the endosome.
Left: Live cell fluorescence microscopy of a HeLa cell showing trafficking of a GFP-tagged secretory protein (Syndecan-1) through the secretory pathway. A protein of the Golgi apparatus appears in magenta. Images by Yeongho Kim, Ph.D.